The Canine Brucella Antibody Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Canine Brucella Antibody (C.Brucella Ab) in dog’s serum, plasma, or whole blood. Assay Time: 5-10 minutes Specimen: serum, plasma, or whole blood.
PRINCIPLE: The Canine Brucella Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Brucella LPS antigens. If there are anti-Brucella antibodies in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Canine Brucella antibodies in the specimen.
*TEST PROCEDURE
* Collect cattle’s fresh whole blood, and get the serum or plasma specimen for use. Immediately apply the testing once collected the specimen. * Take out the test device from the foil pouch and place it horizontally. * Place 3 drops(around 120uL) of serum or plasma specimen into the sample hole. Start timer and wait for result. * Interpret the result in 5-10 minutes. Result after 15 minutes is considered as invalid.
* INTERPRETATION OF RESULTS * Positive (+): The presence of both “C” lineand zone “T” line. Faint results must be classified as positive. * Negative (-): Only clear C line appear. No T line. * Invalid: No colored line appears in C zone. No matter if T line appears.